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KMID : 0880220140520070609
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Journal of Microbiology
2014 Volume.52 No. 7 p.609 ~ p.618
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Genotyping, morphology and molecular characteristics of a lytic phage of Neisseria strain obtained from infected human dental plaque
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Aljarbou Ahmed N.
Aljofan Mohamad
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Abstract
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The lytic bacteriaphage (phage) A2 was isolated from human dental plaques along with its bacterial host. The virus was found to have an icosahedron-shaped head (60±3 nm), a sheathed and rigid long tail (∼175 nm) and was categorized into the family Siphoviridae of the order Caudovirales, which are dsDNA viral family, characterised by their ability to infect bacteria and are nonenveloped with a noncontractile tail. The isolated phage contained a linear dsDNA genome having 31,703 base pairs of unique sequence, which were sorted into three contigs and 12 single sequences. A latent period of 25 minutes and burst size of 24±2 particles was determined for the virus. Bioinformatics approaches were used to identify ORFs in the genome. A phylogenetic analysis confirmed the species inter-relationship and its placement in the family.
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KEYWORD
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Neisseria sp., Siphoviridae, lytic phage A2, human dental plaques, contigs, phylogenetic relationships
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